Isolation and Molecular Characterization of Salmonellae Isolated from Some Meat Products

Document Type : Original Article

Authors

1 department of food hygiene faculty of veterinary medicine university of Sadat city

2 Department of Bacteriology, Mycology and Immunology, Faculty of Veterinary Medicine, University of Sadat City, Egypt

3 Department of Food Hygiene & Control, Faculty of Veterinary Medicine, University of Sadat City, Egypt.

Abstract

A Total of 200 random samples of meat products (minced meat, beef burger, kofta, sausage and luncheon) collected from different shops and supermarkets at El Menofiya Governorate, Egypt. The collected samples were examined for the isolation of Salmonella spp, serological identifications and molecular characterization by using PCR. Salmonella spp was isolated from the examined samples with the percentage of 25% (10) ,35% (14) ,27.5% (11), 30% (12) and 7.5% (3) respectively. The isolated Salmonella serologically identified as S. Enteritidis, S. Infantis, S. Paratyphi A and S. Typhimurium, also multiplex PCR methods were used for detection of virulence genes (invA and hilA ) genes of Salmonella. The PCR results revealed that invasion gene (inv A) and hyper-invasive locus gene (hilA) in S.Entertidis , S. Paratyphia A and S. Typhimurium . While S. Infants only positive for Invasion gene (invA) could be detected.

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