Identification, Serological and Molecular Identification of Coliform Bacteria Recovered from Bovine Mastitis

Mousa, Walid S.; Sabbah, Khaled; Nayel, Mohamed A.; Elsify, Ahmed M.; Elsobky, Yumna; Elgendy, Asmaa Abdel Sadek; Sakr, Moustafa; Salama, Akram A

doi:10.21608/jcvr.2023.320441

Identification, Serological and Molecular Identification of Coliform Bacteria Recovered from Bovine Mastitis

Document Type : Original Article

Authors

10.21608/jcvr.2023.320441

Abstract

Aim/Background: Mastitis is one of the serious disease and critical problem in the dairy
industry worldwide. This study aimed to determine the prevalence of coliform bacteria,
particularly E. coli, associated with mastitis in dairy herds. In addition, serological
identification and molecular detection for E. coli strains from bovine mastitis.
Methods: A total of 400 cows were inspected for clinical and subclinical mastitis, and 100
samples (64 and 36, respectively) from these conditions were collected for bacteriological
identification before being subjected to serological and molecular analysis.
Results: Clinical and subclinical mastitis were prevalent; 16% and 9%, respectively.
Bacteriological examination revealed that E. coli was the most prevalent bacteria 16
isolates (19.51%) followed by Klebsiella spp 8 (9.76%), Citrobacter 4 (8.16%), and
Enterobacter aerogens 2 (4.08%). Regarding to the serological results, E. coli isolates
classified into EHEC which was the most identified serotype followed by EPEC and ETEC
serotypes. While, for Klebsiella spp., Klebsiell aoxytoca and Klebsiella pneumonia were serologically
identified. The molecular detection some of the virulence genes by PCR approach among
E.coli isolates reported that all E. coli strains carried the fimH (100%). Meanwhile, other
virulence genes sxt1 and eaeA gene didn’
t detected in any tested E. coli isolates.
Conclusion: Coliform bacteria were identified as major bacterial pathogens involved in
bovine clinical and subclinical mastitis.

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