Preparation and Conjugation of Staphylococcal protein A and streptococcal protein G with fluorescein isothiocyanate as non-species specific diagnostic kits

Document Type : Original Article

Authors

1 Animal Health research Institute Bacteriology Department-Dokki – Giza – Egypt.

2 Vetrinary Serum and Vaccine Research Institute-Abassia, Cairo - Egypt

3 Animal Reproduction Research Institute, Haram, Giza. Egypt.

Abstract

Staphylococcal protein A “SPA” and streptococcal protein G “SG” are binds to the most mammalian class G immunoglobulin. Therefore “SPA” and “SG” has a unique potential for making “universal” tool, which can be used in various immunoassays which achieve early diagnosis and quick perform till date including coagulation test; Immune fluorescence antibody technique (IFT) and enzyme linked immune sorbent assay (ELISA). In this study “SPA” and “SG” were prepared using two reference strains of Cowan-1 and group C streptococci. Three classical forms of SPA and SG were used including crud; purified and fluorescein isothiocyanate “FITC” conjugates. Such preparations were used in application of coagulation test and fluorescent antibody technique on reference antigens and antibodies (antisera) in order to evaluate their efficacy. The used antisera were obtained from different animal species including cattle; buffaloes; sheep; goat; dogs; horse; rabbit; mice as well as camel sera. SPA and SG also used for differentiation between IgG1 andIgG2 subclass in serum of different animal species. In conclusion it could be stated that SPA and SG are safe; easy to be used, high stable, highly sensitive, can be used as rapid field test, of low cost having friendly environmental relationship.

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