Bacteriological and Molecular Identification of Mycoplasma Bovis and Mycoplasma bovigenitalium Isolated from Cattle and Buffaloes

Rashad, Hesham; Zaghawa, Ahmed; Nayel, Mohamed; Mousa, Walid; Salama, Akram; Eissa, Sabry; Mohamed, Yousreya H.

doi:10.21608/jcvr.2024.384952

Bacteriological and Molecular Identification of Mycoplasma Bovis and Mycoplasma bovigenitalium Isolated from Cattle and Buffaloes

Document Type : Original Article

Authors

10.21608/jcvr.2024.384952

Abstract

This study was intended to detect the most common Mycoplasma species involved in
respiratory diseases in both cattle and buffaloes. In this study, examination of 300 cattle
and 200 buffaloes was carried out and then the collected samples were subjected to
isolation of Mycoplasmaspecies followed by PCR confirmation. The results showed that
the percent of mollicutes isolation from pneumonic lung tissues of cattle& buffaloes was
22% and 17%, respectively. Moreover, the percent of mollicutes isolation from nasal
swabs of diseased cattle & buffaloes was 18% and 20%, respectively. PCR was effective
in the detection of both M. bovis and M. bovigenitalium through successful amplification
of the Mbo and Mbg gene at 360 and 312 bp, respectively. The minimum inhibitory
concentration was performed on two field strains of M. bovis against seven different
antimicrobial agents. The first strain was sensitive to Draxxin® 10 %, Marbocyle® 10%,
Lincospectin®, Duocycline ® 20%, and Tilmicosin® 30%. The second strain was sensitive
to Duocycline® 20% and Tilmicosin® 30% and resistant to other antimicrobial agents.
Regarding the associated risk factors with Mycoplasmosis; the season was significantly
correlated and the infection was 6.5 times more prevalent in the winter season than
summer season. As well as the sex revealed a significant effect and 5.08 times in males
more than females. Additionally, cattle were more susceptible than buffaloes for the
disease prevalence. Meanwhile, the age was not a significant risk for the disease
prevalence.

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