Prevalence and Molecular Phylogeny of Ascaridia galli in Baladi Chickens in Menouf District, Menoufia, Egypt

El- Debakhy, Mona; Elkhatam, Ahmed; Elbahy, Nasr; AbouLaila, Mahmoud; Shehata, Awad A.; Sabra, Ebrahim A.

doi:10.21608/jcvr.2024.384956

Prevalence and Molecular Phylogeny of Ascaridia galli in Baladi Chickens in Menouf District, Menoufia, Egypt

Document Type : Original Article

Authors

10.21608/jcvr.2024.384956

Abstract

Native chickens ascaridiosis (Gallus domesticus) is a disease caused by obligatory
intestinal Ascaridia galli (Family: Ascarididae) that causes more economic losses in the
chicken industry. The current study was conducted in Menouf district, Menoufia, Egypt
from January to December 2022 to determine chicken ascaridiosis’s prevalence and
identify the recovered Ascaridia galli. Intestinal samples of 898 randomly selected from
Baladi chickens were subjected to parasitological examination. The results revealed that
Ascaridia galli worms were detected in 12.5 % (112 out of 898). The prevalence was
significantly influenced by the area of the examined chickens but not by the season.
Morphological description and molecular characterization of recovered Ascaridia galli
were reported in this study to discriminate Ascaridia galli in infected chickens. The PCR
of Cox 1 of Ascaridia galli produced specific bands of 533 bp. The sequence has an
identity percentage of up to 100% with A. galli from Brazil, Denmark, and USA. The
UPGMA phylogenetic tree showed that the sequence of A. galli Cox-1 from Egypt
clustered with A. galli sequences from Brazil, Denmark, and Ghana. This study provides
data on the prevalence and molecular characterization of A.galli from the Menouf district,
Menoufia, Egypt.

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