Impact of Insemination Site Using Frozen-Thawed Semen on the Pregnancy Rate in Mares

Sultan, Mahmoud A.; Zaghloul, Ahmed H. Zaghloul; Abd El-Razek, Emad M.; Elbaz, Hamed T.; Derbala, Mohamed K.

doi:10.21608/jcvr.2025.462663

Impact of Insemination Site Using Frozen-Thawed Semen on the Pregnancy Rate in Mares

Document Type : Original Article

Authors

10.21608/jcvr.2025.462663

Abstract

The aim of the current study was to study the effect of using frozen semen and the effect of the site of insemination on pregnancy rate in mares. Thirty-one mares were used. Two different artificial insemination protocols were carried out. In the first protocol, 12 mares were inseminated in the uterine body using cryopreserved-thawed semen of stallions with good fertility without adding additional extender using insemination pipette and insemination gun. In the second protocol, 19 mares were inseminated in the uterine horn ipsilateral to the ovary from which ovulation had occurred using cryopreserved-thawed semen of stallions of good fertility without adding additional extender using insemination pipette and insemination gun. Thirteen days after insemination, the mares were examined for diagnosis of pregnancy using ultrasonography. Results showed that pregnancy rate in case of insemination into the uterine horn was not significantly (P > 0.05) higher than insemination into the uterine body. In conclusion, artificial insemination of mares deeply into the uterine horn gave statistically better success nearly similar to insemination into the uterine body.

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